chtog cdna Search Results


chtog cdna  (Addgene inc)
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Addgene inc chtog cdna
Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM <t>chTOG,</t> or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.
Chtog Cdna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chtog+cdna/MBP-ch-TOG-His6+(Plasmid+%2369108)/pm37184584-253-3-13
Average 91 stars, based on 1 article reviews
chtog cdna - by Bioz Stars, 2026-10
91/100 stars
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c1orf77 chtop nm 001206612 human tagged orf clone  (OriGene)
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CHTOP Myc DDK tagged Homo sapiens chromatin target of PRMT1 CHTOP transcript variant 2
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c1orf77 chtop nm 015607 human untagged clone  (OriGene)
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CHTOP untagged Human chromatin target of PRMT1 CHTOP transcript variant 1
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c1orf77 chtop nm 015607 human 3 utr clone  (OriGene)
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3 UTR clone of chromosome 1 open reading frame 77 C1orf77 for miRNA target validation
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c1orf77 chtop nm 015607 human tagged orf clone  (OriGene)
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Lenti ORF clone of Human chromatin target of PRMT1 CHTOP transcript variant 1 mGFP tagged
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mouse chtop gene orf cdna clone expression plasmid  (Sino Biological)
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Full length Clone DNA of Mouse chromatin target of PRMT1.
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chtop nm 001293780 mouse untagged clone  (OriGene)
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Chtop untagged Mouse chromatin target of PRMT1 Chtop transcript variant 6
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chtop nm 023215 mouse tagged orf clone  (OriGene)
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Chtop GFP tagged Mouse RIKEN cDNA 2500003M10 gene 2500003M10Rik 10ug
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c1orf77 chtop nm 001244664 human tagged orf clone  (OriGene)
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CHTOP Myc DDK tagged Homo sapiens chromatin target of PRMT1 CHTOP transcript variant 3
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mouse chtop gene orf cdna clone expression plasmid, n-ofpspark tag  (Sino Biological)
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Full length Clone DNA of Mouse chromatin target of PRMT1 with N terminal OFPSpark / RFP tag.
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Image Search Results


Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.

Journal: The Journal of cell biology

Article Title: CLASPs stabilize the pre-catastrophe intermediate state between microtubule growth and shrinkage.

doi: 10.1083/jcb.202107027

Figure Lengend Snippet: Figure 1. CLASP1 promotes the depolymerization of GMPCPP-stabilized microtubules in a GTP-dependent manner. (A) Schematic of the microtu- bule depolymerization assay. (B) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1. (C) Representative kymographs of GMPCPP-stabilized microtubules incubated with storage buffer, 200 nM chTOG, or 200 nM CLASP1 in the presence of 1 mM GTP. (D) Quantification of microtubule depolymerization rates for the conditions in B and C. N = 50–108 microtubules for each condition across at least two experimental days. Individual data points from different experiments are plotted in different shades, and the means for each experimental repeat are plotted as larger points in the same color. The squares indicate the average of the experimental means, and the vertical bars are the standard errors of the means. See also Video 1. For comparison between the no GTP conditions, a one-way ANOVA followed by Tu- key’s HSD test for multiple comparisons found that the mean rate of microtubule depolymerization was significantly different between the buffer control vs. chTOG (P < 0.001) and chTOG vs. CLASP1 (P < 0.001) in the absence of GTP. There was no statistically significant difference between the buffer control and CLASP1 condition (P = 0.06) in the absence of GTP. Welch’s two-tailed unequal variances t tests were performed for pairwise comparison of the no-GTP vs. GTP conditions and the corresponding P values are indicated on the graph.

Article Snippet: The plasmid containing chTOG cDNA was a gift from Stephen Royle (plasmid #69108; Addgene; http://n2t.net/addgene:69108; RRID: Addgene_69108).

Techniques: Incubation, Comparison, Control, Two Tailed Test